Synergistic antitumor effects of 5-FU and Apatinib on papillary thyroid cancer in vitro and in vivo

Introduction Papillary thyroid carcinoma (PTC) is the most common type of malignancy with increased incidence rapidly occurring in countries. The study aimed to investigate antitumor effects combination 5-FU and Apatinib on PTC. Material methods TPC-1 SW579 cells were treated 5-FU, Apatinib, or a Apatinib. Cell viability was assessed by CCK-8 assay. Edu staining colony formation used evaluate cell proliferation ability. Flow cytometry analyze apoptosis. Wound healing Transwell assay conducted migration invasion. angiogenesis conducting tube Xenograft tumor models established injecting into nude mice. Expressions CD31, VEGFA, VEGFR2 determined using immunofluorescence Western blotting. Results Compared treatment alone, produced stronger suppressive proliferation, migration, invasion, angiogenesis. An vivo experiment showed that strongly suppressed growth. remarkably expressions VEGFR2, associated Conclusions Our data demonstrated combined therapy obtained synergistic PTC, compared either alone down-regulating VEGFA/VEGFR2 signaling pathways.